# DrDimont: Drug Response Prediction from Differential Multi-Omics Networks

## Introduction

The main purpose of the pipeline is to easily and efficiently generate, reduce, and combine molecular networks from two groups or conditions (e.g., of patients) to compute a differential drug interaction score based on drug targets. This allows for improved predictions of the effect of drugs (e.g., for cancer) on two groups with different characteristics.

## Installation

The R package DrDimont can be installed via CRAN or with remotes::install_gitlab("PHiort/DrDimont"). The requirements of the package will be installed when installing the package. The complete source code can be accessed through https://gitlab.com/PHiort/DrDimont.

library(DrDimont)

### Installation of Python dependencies

The pipeline uses a Python script for an intermediate step. For differential drug response score computation, Python (>= 3.8) needs to be installed on the system. The install_python_dependencies() function of DrDimont can be used to install the Python dependencies. Depending on which Python package manager is installed on your system, the dependencies can be installed using pip (default):

install_python_dependencies(package_manager="pip")

or conda:

install_python_dependencies(package_manager="conda")

With conda the python libraries will be installed in the base environment or in the currently active environment if run via command line.

Alternatively the Python dependencies can be installed manually using the DrDimont/inst/requirements_pip.txt or the DrDimont/inst/requirements_conda.txt file.

## Example Data Set Description

The following exemplary pipeline application showcases the usage of molecular breast cancer data with ER+ (Estrogen receptor-positive) patient samples as group A and ER- (Estrogen receptor-negative) as group B. A reduced exemplary data set is included within the package.

[image created with biorender.com]

The breast cancer data by Krug et al. (2020) used for this tutorial is already preprocessed and only includes samples with tumor purity > 0.5 and known ER status. Metabolite data was sampled randomly to generate distributions similar to those reported, e.g., in Terunuma et al. (2014). Krug et al. (2020) published data from the Clinical Proteomic Tumor Analysis Consortium (CPTAC).

The data set contains observations from:

• 78 ER+ samples
• 34 ER- samples
Number of genes, etc. Preprocessing Identifier
mRNA 13915 quantified mRNA expression; log2-transformed FPKM values, NAs set to -11, removed mRNAs with > 90% of zero measurements, reduced gene name
Protein 5809 (ER+) and 5845 (ER-) quantified proteomics data; normalized, standardized, removed proteins with > 20% NAs, reduced NCBI RefSeq ID, gene name
phosphosites 10272 (ER+) and 11318 (ER-) quantified phosphoproteomics data; normalized, removed phosphosites with > 20% NAs, reduced phosphosite, gene name, NCBI RefSeq ID
Metabolite 275 from 33 (ER+) and 34 (ER-) samples randomly sampled metabolomics data; removed metabolites with > 50% NAs biochemical name, PubChem ID, metabolon ID

To limit run time and space requirement of the example we reduced the mRNA, protein and phosphosite data to a random set of 50 genes. The 50 genes were randomly selected from the set of genes with known drug targets from The Drug Gene Interaction Database (https://www.dgidb.org/). The metabolite data was also randomly reduced to 50 metabolites.

First you load the pre-processed data. This data is included in the package and does not need to be manually loaded but can be directly accessed once library(DrDimont) is called.

data("mrna_data")
data("protein_data")
data("phosphosite_data")
data("metabolite_data")
data("metabolite_protein_interactions")
data("drug_gene_interactions")

### Transforming the data to the required input format

After loading the data, you can use formatting functions to bring the data into the required input format:

• make_layer() # Creates individual molecular layers from raw data and unique identifiers
• make_connection() # To specify connections between two individual layers
• make_drug_target() # Formats drug target interactions

#### Create individual layers data structure from the molecular data

Before running the pipeline, individual layer objects have to be created using make_layer(). The user should supply raw data stratified over two patient groups and unique identifiers for the molecular entities, e.g, genes. The function make_layer() requires these input: name, data_groupA, data_groupB, identifiers_groupA and identifiers_groupB. A layer should be given a unique name with the name argument. The identifiers_groupA and identifiers_groupB should be data frames containing one or more uniquely named columns with identifiers of the molecular entities, e.g., gene names. The raw data for data_groupA and data_groupB should be given with the samples as rows and the molecular entities (e.g, genes) as columns. The identifiers of the molecular entities need to be in the same order as the columns in the raw data. If you have only one group to analyse then set the parameters data_groupB=NULL and identifiers_groupB=NULL.

Run the code below for exemplary raw data frames:

# Data inspection
mrna_data$groupA[1:3, 1:5] #> gene_name X01BR015 X01BR018 X01BR023 X01BR025 #> 1 ABCA1 2.6616 3.0655 2.4372 2.0085 #> 2 CACNA2D1 -0.0491 2.4549 0.4724 -2.6449 #> 3 CASP3 3.9785 4.5653 3.3765 3.7385 protein_data$groupA[1:3, 1:5]
#>       ref_seq gene_name X01BR015 X01BR018 X01BR023
#> 1 NP_004360.2    COL6A3   2.8159  -1.0654  -0.1252
#> 2 NP_476507.3    COL6A3   0.7548  -2.8463   0.3242
#> 3 NP_005600.1      PYGM   0.4418   0.6022   0.5072
phosphosite_data$groupA[1:3, 1:5] #> site_id gene_name ref_seq X01BR015 X01BR018 #> 1 NP_001006666.1_S230s _1_1_230_230 RPS6KA1 NP_001006666.1 0.4518 0.0663 #> 2 NP_001006666.1_S372s _1_1_372_372 RPS6KA1 NP_001006666.1 0.1946 -0.4792 #> 3 NP_001006666.1_S389s _1_1_389_389 RPS6KA1 NP_001006666.1 -0.7127 -0.1509 metabolite_data$groupA[1:3, 1:5]
#>                   biochemical_name metabolon_id pubchem_id       X1        X2
#> 2             1-methylnicotinamide        27665        457  61561.9  38714.07
#> 4 1-stearoylglycerophosphoinositol        19324       6563 106015.4        NA
#> 6     cytidine 5'-diphosphocholine        34418      13804       NA 736205.19

With the code below we create the individual layers:

# Create individual layers
mrna_layer <- make_layer(name="mrna",
data_groupA=t(mrna_data$groupA[,-1]), data_groupB=t(mrna_data$groupB[,-1]),
identifiers_groupA=data.frame(gene_name=mrna_data$groupA$gene_name),
identifiers_groupB=data.frame(gene_name=mrna_data$groupB$gene_name))
#> [22-05-13 20:59:01] Layer mrna, groupA contains 78 samples and 50 genes/proteins/entities.
#> [22-05-13 20:59:01] Layer mrna, groupB contains 34 samples and 50 genes/proteins/entities.

protein_layer <- make_layer(name="protein",
data_groupA=t(protein_data$groupA[, c(-1,-2)]), data_groupB=t(protein_data$groupB[, c(-1,-2)]),
identifiers_groupA=data.frame(gene_name=protein_data$groupA$gene_name,
ref_seq=protein_data$groupA$ref_seq),
identifiers_groupB=data.frame(gene_name=protein_data$groupB$gene_name,
ref_seq=protein_data$groupB$ref_seq))
#> [22-05-13 20:59:01] Layer protein, groupA contains 78 samples and 53 genes/proteins/entities.
#> [22-05-13 20:59:01] Layer protein, groupB contains 34 samples and 53 genes/proteins/entities.

phosphosite_layer <- make_layer(name="phosphosite",
data_groupA=t(phosphosite_data$groupA[, c(-1,-2, -3)]), data_groupB=t(phosphosite_data$groupB[, c(-1,-2, -3)]),
identifiers_groupA=data.frame(phosphosite_data$groupA[, 1:3]), identifiers_groupB=data.frame(phosphosite_data$groupB[, 1:3]))
#> [22-05-13 20:59:01] Layer phosphosite, groupA contains 78 samples and 61 genes/proteins/entities.
#> [22-05-13 20:59:01] Layer phosphosite, groupB contains 34 samples and 65 genes/proteins/entities.

metabolite_layer <- make_layer(name="metabolite",
data_groupA=t(metabolite_data$groupA[, c(-1,-2, -3)]), data_groupB=t(metabolite_data$groupB[, c(-1,-2, -3)]),
identifiers_groupA=data.frame(metabolite_data$groupA[, 1:3]), identifiers_groupB=data.frame(metabolite_data$groupB[, 1:3]))
#> [22-05-13 20:59:01] Layer metabolite, groupA contains 33 samples and 50 genes/proteins/entities.
#> [22-05-13 20:59:01] Layer metabolite, groupB contains 34 samples and 49 genes/proteins/entities.

Below we are creating a list of all individual layers for pipeline input:

all_layers <- list(mrna_layer, protein_layer, phosphosite_layer, metabolite_layer)

#### Create inter-layer connections data structure

The inter-layer connections need to supplied by the user with make_connection(). The parameters from and to have to match to a name in the previously created layers by make_layer(). The established connection will result in an undirected combined graph. There are two options to connect layers: (i) based on identical identifiers of entities, or (ii) based on a given interaction table.

For (i), two layers have to have one matching column name in their identifier data frame that is passed as the parameter connect_on. Two entities in the different layers with the same ID therein are connected with an edge of fixed weight (indicated by the weight parameter, default 1).

For example:

# (i) make inter-layer connection
make_connection(from='mrna', to='protein', connect_on='gene_name', weight=1)

For (ii), an interaction table containing three columns is required. Two columns should contain entity IDs that are also given in the respective identifier data frames of the two layers to be connected. One column of those should be named the same as a column name given in the identifier data frame of one layer and the second column the same for the second layer. The third column should contain the weights with which the respective entities of the two layers should be connected.

See data(metabolite_protein_interactions) for an exemplary interaction table. The table contains the columns pubchem_id also given for the metabolite layer, gene_name also given for the protein layer, and combined_score containing the weights for the respective interactions:

# Data inspection
metabolite_protein_interactions[1:3, ]
#>   pubchem_id gene_name combined_score
#> 1       1117      ARF5          0.942
#> 2   20042692      ARF5          0.933
#> 3     444727      M6PR          0.957

The interaction table should be passed to the connect_on parameter of the function make_connection() and the column name of the column containing the weights to the weight parameter.

For example:

# (ii) make inter-layer connection
make_connection(from='protein', to='metabolite', connect_on=metabolite_protein_interactions, weight='combined_score')

If you have only one layer you can skip this step and set the parameter inter_layer_connections=NULL later on (see: Running the complete pipeline).

Below we are creating a list of all inter-layer connections for pipeline input:

all_inter_layer_connections = list(
make_connection(from='mrna', to='protein', connect_on='gene_name', weight=1),
make_connection(from='protein', to='phosphosite', connect_on='gene_name', weight=1),
make_connection(from='protein', to='metabolite',
connect_on=metabolite_protein_interactions, weight='combined_score')
)

#### Create drug-target interaction data structure

To run the entire pipeline, drug-target interactions are required. For that we need an interaction table mapping drugs to their targets, e.g, proteins. The table should contain two columns: one column containing the drug ids with the name drug_name and another column containing the drug targets with a name matching a column name in the identifier data frame of the target layer. In our example we are using a table from The Drug Gene Interaction Database providing interactions of drugs with genes. The exemplary data frame has three columns (gene_name, drug_name, drug_chembl_id), one containing the gene names also given for the target protein layer, the second containing the drug names which are used to identify the drugs and a third column containing the ChEMBL IDs of drugs which will be ignored in the pipeline.

For example:

# Data inspection
drug_gene_interactions[1:3, ]
#>   gene_name   drug_name drug_chembl_id
#> 1      CDK7  BMS-387032   CHEMBL296468
#> 3      APOE  PREDNISONE      CHEMBL635

The function make_drug_target() generates the required format of the drug-target interactions for the pipeline. The parameter target_molecules should match one of the layer names, e.g., protein. The data frame supplied with the parameter interaction_table should map drugs to their target as described above. The column in the interaction table containing the targets should be given with the match_on parameter, e.g, match_on=gene_name for protein as targets.

all_drug_target_interactions <- make_drug_target(
target_molecules='protein',
interaction_table=drug_gene_interactions,
match_on='gene_name')

#### Check input data structures

When the input data structures of the individual layers, the inter-layer connections, and the drug target interactions are created they are checked automatically for validity. Additionally, the function below checks for a variety of possible input formatting errors and reports registered data set sizes (samples, entities) for the user to compare with the intended input.

return_errors(check_input(all_layers, all_inter_layer_connections, all_drug_target_interactions))
#> [22-05-13 20:59:02] Layer mrna, groupA contains 78 samples and 50 genes/proteins/entities.
#> [22-05-13 20:59:02] Layer mrna, groupB contains 34 samples and 50 genes/proteins/entities.
#> [22-05-13 20:59:02] Layer protein, groupA contains 78 samples and 53 genes/proteins/entities.
#> [22-05-13 20:59:02] Layer protein, groupB contains 34 samples and 53 genes/proteins/entities.
#> [22-05-13 20:59:02] Layer phosphosite, groupA contains 78 samples and 61 genes/proteins/entities.
#> [22-05-13 20:59:02] Layer phosphosite, groupB contains 34 samples and 65 genes/proteins/entities.
#> [22-05-13 20:59:02] Layer metabolite, groupA contains 33 samples and 50 genes/proteins/entities.
#> [22-05-13 20:59:02] Layer metabolite, groupB contains 34 samples and 49 genes/proteins/entities.

## Running the complete pipeline

The pipeline can be run entirely or in individual steps. To set global pipeline options a settings list needs to be created using the drdimont_settings() function. This function contains default parameters that can be modified as shown below. For a detailed explanation of all possible settings and parameters please refer to the function documentation by calling ?drdimont_settings().

Please be aware of the python script used in one of the pipeline steps (see Requirements above). If you have installed python and the required packages via pip then you should set the drdimont_settings() parameter python_executable="python" or python_executable="python3" depending on your installed python version. If you have installed python and the required packages manually in a custom conda environment then set the drdimont_settings() parameters conda=TRUE and python_executable="-n name-of-your-environment python". If you are using the conda base environment then you can set python_executable="python".

The intermediate pipeline and drug response scores output (parameter save_data) is deactivated for this example (see below) but especially for large data files consider turning it on (the default). You can specify the output location of files with the saving_path parameter. If not specified all files will be written to the current working directory. For the this example, the data will be saved in a temporary directory. If you want to save the data elsewhere you need to change the parameter saving_path below. The intermediate output data includes RData-files of the correlation matrices, the individual graphs, the combined graphs, the drug target edges, the interaction score graphs, and the differential score graph. The drug response scores are saved in a tsv-file in the specified output directory if save_data=TRUE.

The settings list for the example (call ?drdimont_settings() for further information on the parameters):

example_settings <- drdimont_settings(
handling_missing_data = list(
default = "pairwise.complete.obs",
mrna = "all.obs"
),
reduction_method = "pickHardThreshold",
r_squared=list(default=0.65, metabolite=0.1),
cut_vector=list(default=seq(0.2, 0.65, 0.01)),
save_data = FALSE,
python_executable = "python3",
saving_path = tempdir())
# disable multi-threading for example run;
# not recommended for actual data processing
#> 

To run the entire pipeline from beginning-to-end the run_pipeline() function can be used:

run_pipeline(layers=all_layers,
inter_layer_connections=all_inter_layer_connections,
drug_target_interactions=all_drug_target_interactions,
settings=example_settings)

## Running the individual pipeline steps

The pipeline can also be used in a modular fashion. The modules then refer to the different steps:

1. Compute correlation matrices
2. Generate individual graphs
3. Combine graphs
4. Identifying drug targets and their edges
5. Calculate integrated interaction score
6. Generate differential graph
7. Calculate differential drug response score

[image created with biorender.com]

### Step 1: Compute correlation matrices

In step one, correlation matrices are computed for the specified layers described above. spearman (default), pearson, and kendall can be chosen as correlation methods by setting the parameter reduction_method in drdimont_settings(). The list of layers and the settings list are passed to compute_correlation_matrices(). To reduce run time this example will only analyze the first 10 genes and patients of the mRNA layer (set layers=all_layers in compute_correlation_matrices() to compute all layers):

reduced_mrna_layer <- make_layer(name="mrna",
data_groupA=t(mrna_data$groupA[1:10,2:11]), data_groupB=t(mrna_data$groupB[1:10,2:11]),
identifiers_groupA=data.frame(gene_name=mrna_data$groupA$gene_name[1:10]),
identifiers_groupB=data.frame(gene_name=mrna_data$groupB$gene_name[1:10]))

example_correlation_matrices <- compute_correlation_matrices(
layers=list(reduced_mrna_layer),
settings=example_settings)

The resulting data structure example_correlation_matrices is a nested named list with 3 levels containing the correlation matrices and annotation data frames. The first level are correlation_matrices and annotations. The correlation matrices are separated on the second level by group (groupA and groupB) and on the third level by layer name (e.g., mrna, protein, etc.). The annotations element contains annotations for each groups and both combined at the second level (groupA, groupB, andboth). The third level consist of named data frames, for each layers one data frame, which contain the pipeline-internal mapping of the identifier data frames of the individual layers to layer specific node IDs.

For example:

# Data inspection
data("correlation_matrices_example")
correlation_matrices_example$annotations$groupA$protein[1:3, ] #> gene_name ref_seq node_id layer #> 1 COL6A3 NP_004360.2 protein_1 protein #> 2 COL6A3 NP_476507.3 protein_2 protein #> 3 PYGM NP_005600.1 protein_3 protein ### Step 2: Generate individual graphs Next, the individual graphs are generated. In this step edge weights are established based on the correlation computation and the edges are reduced by the specified reduction method. Reduction can be done based on maximizing scale-freeness employing WGCNA::pickHardThreshold (reduction_method=pickHardThreshold in drdimont_settings(); default) or based on significance of the correlation (reduction_method=p_value). data("correlation_matrices_example") example_individual_graphs <- generate_individual_graphs( correlation_matrices=correlation_matrices_example, layers=all_layers, settings=example_settings) The resulting data structure example_individual_graphs is a nested named list with 3 levels containing the graphs and annotation data frames. The element graphs contains, similar to the correlation matrices, the two groups on the second level and the graphs as iGraphs objects for each molecular layer on the third level. The annotations element is a copy from the annotations element of the example_correlation_matrices data (see Step 1). ### Step 3: Combine graphs In this step, the individual graphs are combined to a single combined graph per group based on the inter-layer connections created above. The function creates the disjoint union of the individual graphs and adds inter-layer edges with the specified weight. example_combined_graphs <- generate_combined_graphs( graphs=example_individual_graphs[["graphs"]], annotations=example_individual_graphs[["annotations"]], inter_layer_connections=all_inter_layer_connections, settings=example_settings) #> [22-05-13 20:59:07] Connecting mrna and protein by id. #> [22-05-13 20:59:07] groupA: Generating inter-layer edgelist... #> [22-05-13 20:59:07] done. #> [22-05-13 20:59:07] groupB: Generating inter-layer edgelist... #> [22-05-13 20:59:07] done. #> [22-05-13 20:59:07] Connecting protein and phosphosite by id. #> [22-05-13 20:59:07] groupA: Generating inter-layer edgelist... #> [22-05-13 20:59:07] done. #> [22-05-13 20:59:07] groupB: Generating inter-layer edgelist... #> [22-05-13 20:59:07] done. #> [22-05-13 20:59:07] Connecting protein and metabolite by table. #> [22-05-13 20:59:07] groupA: Generating inter-layer edgelist... #> [22-05-13 20:59:07] done. #> [22-05-13 20:59:07] groupB: Generating inter-layer edgelist... #> [22-05-13 20:59:07] done. #> [22-05-13 20:59:07] Combining graphs... #> [22-05-13 20:59:07] groupA... #> [22-05-13 20:59:07] groupB... #> [22-05-13 20:59:07] groupB done. The resulting data structure example_combined_graphs is a nested named list with 2 levels containing the combined graphs and a combined annotation data frame. The element graphs contains the two groups on the second level with the combined graphs as iGraphs objects. The annotations element consists of a data frame on the second level named both which contains the mapping of the identifier data frames of the individual layers to the layer specific node IDs for all layers together. For example: # Data inspection example_combined_graphs$annotations\$both[1:3, ]
#>   gene_name node_id layer ref_seq site_id biochemical_name metabolon_id
#> 1     ABCA1  mrna_1  mrna    <NA>    <NA>             <NA>           NA
#> 2  CACNA2D1  mrna_2  mrna    <NA>    <NA>             <NA>           NA
#> 3     CASP3  mrna_3  mrna    <NA>    <NA>             <NA>           NA
#>   pubchem_id
#> 1         NA
#> 2         NA
#> 3         NA

### Step 4: Identifying drug targets and their edges

Next, in order to extract the list of relevant drugs the drug targets are identified in the combined graph for each group. Here, the node IDs of the specified drug targets are found in the combined graph and the drugs are mapped to their target nodes. Additionally, edge lists are returned containing the incident edges of drug target nodes for which integrated interaction scores are computed in the next step.

example_drug_target_edges <- determine_drug_targets(
graphs=example_combined_graphs[["graphs"]],
annotations=example_combined_graphs[["annotations"]],
drug_target_interactions=all_drug_target_interactions,
settings=example_settings)
#> [22-05-13 20:59:07] Determining drug targets...
#> [22-05-13 20:59:08] 53 drug targets found.
#> [22-05-13 20:59:08] 261 drugs with targets found.
#> [22-05-13 20:59:08] done.

The resulting data structure example_drug_target_edges is a nested named list with 2 levels. The first level consists of the elements targets and edgelists. The element targets contains the data frame target_nodes and the dictionary-like list drugs_to_target_nodes. The data frame target_nodes contains the node IDs of the nodes that are drug targets and TRUE/FALSE values if they are present in the graph of each group. The list drugs_to_target_nodes maps the drugs to the node IDs of their targets. The element edgelists consists of a data frame for each of the groups (groupA and groupB) which, respectively, contain the incident edges of the drug targets and their weights (columns from, to and weight).

### Step 5: Calculate integrated interaction score

In this step, the combined graphs for each group together with the edge list from drug_target_edges are used to calculate the integrated interaction scores for all edges incident to drug targets. The pipeline uses a Python script to compute the integrated interaction scores in the function generate_interaction_score_graphs(). The input data for the Python script (combined graphs for both groups in gml format and relevant edges lists for both groups in tsv format) are written to disk and the script is called to calculate the scores. Output files written by the Python script are two graphs in gml format containing the interaction score as an additional edge attribute called interactionweight. These are then loaded into R and returned in a named list containing the graphs for groupA and groupB, respectively.

Attention : Data exchange via files is mandatory and can take long for large data. Additionally, the interaction score computation can be slow. Therefore, do not set max_path_length in drdimont_settings() to a large value (default: 3). If max_path_length=1 then the integrated interaction scores will be the same as the correlation-based edge weights.

The Python script for integrated interaction score computation is parallelized using ray (https://www.ray.io/). Refer to the Ray documentation if you encounter problems with running the python script in parallel. Use the setting int_score_mode="sequential" in drdimont_settings() for forced sequential computation or int_score_mode="ray" for parallel computation otherwise one of the two will be automatically chosen based on the size of the data.

Running Ray on a compute cluster : If you want to run DrDimont on a cluster, run ray start --head --num-cpus 12 (change number of CPUs as fit) on the command line, before starting the R session.

example_interaction_score_graphs <- generate_interaction_score_graphs(
graphs=example_combined_graphs[["graphs"]],
drug_target_edgelists=drug_targets[["edgelists"]],
settings=example_settings)

### Step 6: Generate differential graph

To generate a differential graph, the difference of the interaction scores between the two groups is computed by subtracting the values of the edge attributes of groupB from groupA (i.e., groupA - goupB). A single differential graph with differential_score and differential_interaction_score as edge attributes is returned. The edge attribute differential_score is the difference of the correlation-based edge weights and differential_interaction_score is the difference in integrated interaction scores. Missing edges in one of the two groups are set to zero before computing the difference. The differential integrated interaction score is set to be NA if the integrated interaction score was not computed in both groups, i.e., for all edges not incident to a drug target.

data("interaction_score_graphs_example")
example_differential_graph <- generate_differential_score_graph(
interaction_score_graphs=interaction_score_graphs_example,
settings=example_settings)
#> [22-05-13 20:59:08] Computing differential networks...
#> [22-05-13 20:59:08] done.
#if interaction score graphs were computed use the following:
#example_differential_score_graph <- generate_differential_score_graph(
#                                           interaction_score_graphs=example_interaction_score_graphs,
#                                           settings=example_settings)

### Step 7: Calculate differential drug response score

In the last step, the differential drug response score is calculated based on the differential graph. The score of a drug is the mean (default) or the median of all differential integrated interaction scores of the edges incident to the drug targets. Drugs that have only targets without any edges have a NA as differential drug response.

example_drug_response_scores <- compute_drug_response_scores(
differential_graph=example_differential_graph,
drug_targets=example_drug_target_edges[["targets"]],
settings=example_settings)
#> [22-05-13 20:59:08] Computing drug response scores based on the mean of the differential scores ...
#> [22-05-13 20:59:08] done.

The first few lines of the resulting data frame are shown below. The data frame is saved as drug_response_score.tsv in the specified output folder (see above). The drug response score is an indirect measure of how the strength of connectivity differs between the groups for the drug targets of the particular drug.

head(dplyr::filter(example_drug_response_scores, !is.na(drug_response_score)))
#>                     drug_name drug_response_score
#> 1 (7S)-HYDROXYL-STAUROSPORINE           0.1805916
#> 2                 ABEMACICLIB           0.2581332
#> 3               ACALABRUTINIB           0.2378911
#> 5                    AFATINIB           0.2581332
#> 6                     ALCOHOL           0.0000000

## References

Full citation:

Krug et. al (2020):

Terunuma et al.(2014):

• Terunuma, Atsushi et al. “MYC-driven accumulation of 2-hydroxyglutarate is associated with breast cancer prognosis.” The Journal of clinical investigation vol. 124,1 (2014): 398-412. https://www.doi.org/10.1172/JCI71180

The package DrDimont is an updated version of the previously published molnet package (https://github.com/molnet-org/molnet; https://CRAN.R-project.org/package=molnet)